Paper showing that injury to the visual brain can lead to detectable changes in the eyes

Paper showing that injury to the visual brain can lead to detectable changes in the eyes.

New discovery about how the eyes are affected by brain injury could lead to simple non-invasive eye test to detect CVI.

This important paper looks at how injury to the brain may be detected by looking very carefully at the eyes. The researchers have found out how the eyes have been affected in a group of young adults with very early brain injury causing lower visual field impairment.

The relationship between OVI and CVI

On this website we have treated the ocular visual impairments (OVI), that is visual impairments due to damage to the eye, as being different to the cerebral visual impairments (CVI), and this is because the two broad groups are very different in their outcome. They often need very different approaches to support affected people and to make things accessible to learn (as we explained in our introduction to a paper considering OVI versus CVI).

The relationship between OVI and CVI is not 'them and us' though, partly because many people have both CVI and OVI, but also because the two are connected. This connection however is not a simple straight line from one to the other. The OVIs and CVIs are more like living in a neighbourhood, connected by shared pathways, drives and roads.

This paper looks at the whole process, from the eye, to the primary visual cortex in occipital lobes in the brain (known as the area V1 in the brain) and reaches some fascinating and extremely important conclusions.

This paper is written with the expectation that the reader has an understanding of how vision is processed both in the eye and brain.

Below is a very simple explanation; there are many resources on the internet with more comprehensive and detailed explanations, including on the Scottish Sensory Centre website, click here for link to resource.

The Eye

You may have heard the expression 'scratched cornea'. Your cornea is the clear transparent part at the front of your eye that serves two purposes, one to protect, and the other to start to focus the incoming light. Imagine what you are looking at, what is happening is that your cornea bends the incoming light, and directs it through your pupil to a lens behind the pupil. This focuses the light further, onto the back of your eye, to create a picture on the lining at the back (the retina), of what you are looking at.

The fovea is the central part of the retina. This is the part of the eye where the fine visual details of what you are directly looking at are created, so that you can see colours distinctly and can see things clearly, even if they are very small. Just like a video camera, the whole retina converts the picture into electrical signals, and sends this visual information to the occipital lobes (see Eye to Visual Brain, below).

So, the back of your eyeball (on the inside) is lined by tissue called the retina. The retina is made up of several very thin layers. The inner or top layer of the retina is made up of fine silk like nerve fibres (called the retinal nerve fibre layer). The next layer out, is called the ganglion cell layer with each ganglion cell giving off one of these nerve fibres that goes along the optic nerve straight to the brain.

These are the eye structures discussed in this paper.

Gordon N Dutton, Vision and the Brain, AFB Press, 2015

Eye to Visual Brain

The visual information from each eye travels along the optic nerve, to a junction point called the optic chiasm, where it splits into two optic tracts one taking the information about the right side of the scene (from both eyes) to the left side of your brain, the other, taking the visual information about the left side of the scene (from both eyes) to the right side of the brain.

At the end of each optic tract, on both sides, is a relay station inside the brain called the lateral geniculate body, and here the visual information is relayed into the optic radiations, that take the visual image information to the occipital lobes, which assemble the image of what you are looking at, all so quickly that you are unaware the process is even taking place.

Gordon N Dutton, Vision and the Brain, AFB Press, 2015

The optic radiation on each side splits into two on each side, with the information from the upper part of the scene travelling through the temporal lobes, while the information from the lower part of the scene travels through the posterior parietal lobes (shown by the curvey grey arrows in the diagram below).

If the left occipital lobe is injured, the area to the right is not seen, and if the right occipital lobe is injured the area to the left is not seen. Similarly, the upper optic radiations can be injured (for example following damage to the white matter that sometimes affects prematurely born children). This causes lack of vision down below, which is very similar in each eye. We know this as a lower visual field impairment, which we know is common in those who also have dorsal stream dysfunctions, and Balint Syndrome.

Diagram showing how injuries to different parts of the occipital lobes affect vision.Diagram showing how injuries to different parts of the occipital lobes affect vision.Gordon N Dutton, Vision and the Brain, AFB Press, 2015

The Paper

This paper uses the term 'immature optic radiation'. Immature means young or not fully formed, because the people in this study were all born prematurely (although are now adults).

The human brain is typically made up of grey and white matter. The grey matter is on the outside, the white matter on the inside.

Where babies are born prematurely, there can be complications where there is white matter damage (called white matter damage of immaturity, WMDI in this paper). This can lead to lesions of white matter in the brain.

The term lesion in this paper refers to a focus of brain tissue injury.

There are different causes of WMDI. It can happen while the foetus is in the womb, or after the baby is born. The most common form of WMDI is a condition called periventricular leukomalacia.

A common feature of WMDI is lower visual field impairment, caused by damage to the upper (superior) optic radiations.

WMDI can be detected using MRI imaging.

This study uses two different types of test, one an MRI, the other an imaging test called optical coherence tomography (OCT). OCT is a harmless test, using light waves, but provides images of the 3D structure of the retina at a much higher resolution than MRI. Using OCT, the different layers of the retina can be clearly seen.

This research group had found in a previous study that young adults who were born prematurely, who had both a lower visual field impairment and WMDI, also had a thinner (than typical) retinal nerve fibre layer. The thinner retinal nerve fibre layer was considered the secondary outcome of the brain ijury. The primary (main) cause being damage to the upper optic radiations, running through the posterior parietal lobes on their way to the occipital lobes.

  • Question: Why are the posterior parietal lobes affected when there is this type of white matter damage?
  • Answer: The posterior parietal lobes receive their blood supply from three separate sources - the anterior (front), middle and posterior (back) cerebral arteries. This is called a 'triple watershed zone'. Put simply, the area is more likely to be damaged by reduced blood flow than other areas during early development.

This study uses OCT technology to measure the thickness of each layer of the retina, focussing on the macular ganglion cell layer, to see if it is atypical in any way in those born prematurely, who have WMDI and a lower visual field impairment in each eye.

The first challenge was to recruit participants, and of thirty who met the criteria, only eight were asked, because the other twenty two would not have had the 'ability to cope with the demanding MRI examination'.

Anyone who has had an MRI scan will know that they can be stressful, because the patient is in a confined space, must stay very still, and it is VERY LOUD! More profoundly affected children may need to have a general anaesthetic to have an MRI.

The participants also have to be able to follow instructions (and so communicate verbally). This is why most research into the cerebral visual impairments, has been on the more able, and why many of the diagnostic criteria are based on the patient being able to follow instructions, like 'look at that'.

It is normal for studies such as this, to match the patients, one to one, with 'typical' individuals who are similar in age and sex, so that comparisons can be made and measured if possible. This group of people who are matched by age and sex are called the control group.

In total seven patients participated, all were known to have WMDI, but are now adults. None of the seven patients had a history of damage to the structure of their eyes due to their prematurity.

The optic radiations were measured for each participant, and good results were achieved.

The researchers mapped the V1 area of the brain on all the study participants - to do this they used tests covering one eye at a time, and looked at the corresponding area of brain activity, and from this could map the primary visual cortex in the occipital lobe (V1) on each side. This area was typical for all participants, both patients and the control group. V1 includes the outer grey matter / inner white matter areas of the brain.

Once V1 had been identified for each patient, the borders between the dorsal and ventral parts were also defined as the boundary.

Figure 1

These images are of a typical brain.

Figure 1 (the very bright two brains) shows the brain from the two (left and right) sides, with the area V1 marked out. V1v is the ventral border, and from here information would flow along the ventral stream to the temporal lobe. V1d notes the dorsal border, and from here information would flow up to the posterior parietal lobes.

In the other images, the tract of the optic radiation has been coloured:

  • Pink is the optic radiation entering the occipital lobes from the parietal lobes through the dorsal border
  • Turquoise is optic radiation entering the occipital lobes from the temporal lobes through the ventral border.

The participants also undertook visual field tests, and their visual fields were plotted graphically. Using OCT they each had the thickness of their macular ganglion cell layer measured (this measurement was part of an equation, used to calculate a ratio which could be used as a more accurate measurement for comparison purposes).

The patient group had thinner macular ganglion cell layers in the upper retina than the control group.

Figure 2

These images are fascinating!

There are three rows of pictures for three patients A, B & C.

  • The black images on the left are of the brain, and show the optic radiation, coloured pink and turquoise, to separate those destined for the ventral boundary and those destined for the dorsal boundary.
  • On the right, the top (red and yellow) images are the results of the OCT test showing the thickness of the macular ganglion cell layer. Red is thinnest, yellow thinning, grey typical.
  • Below on the right, are the visual field test results for each individual, the darker areas showing where there is visual field loss.

Looking at all the images on the right it is easy to see that patient C is much less affected, by both visual field loss and thinning of the macular ganglion cell layer.

Look at the images on far the left. With patient C, the pink and turquoise are very clearly defined. Now look at A and B, the pink has sort of been absorbed by the turquoise.

What does this all mean?

  • Lower visual field impairment is accompanied by lack of nerve fibres going into the upper (dorsal) occipital lobes, as expected from figure 2.
  • Moreover, this is accompanied by lack of ganglion cells in the upper retina near the centre in both eyes.

This paper raises many questions:

  • No obvious eye damage was present in the patient group, however this level of testing (OCT) had not been invented when the patients, some now in their thirties, were infants. It is highly likely though, that the thinner macular ganglion cell layer has been present from birth or very early childhood. This is probably due to degeneration of all the ganglion cells whose nerve fibres had no nerve fibres in the brain to link up with, owing to the white matter injury.
  • Many people affected by the CVIs have a lower visual field impairment, due to injury of the optic radiations passing through the posterior parietal lobes, for example from genetic disorders affecting foetal brain development, or oxygen starvation due to birth complications. It would be useful to see if the same pattern of retinal ganglion cell injury occurs in these cases.
  • For the more profoundly affected, unable to endure the tests, is there a way of seeing if the same is true?
  • Amongst the least profoundly affected, might this OCT test give an indication of CVI?
  • With acquired CVIs, for example through a stroke, over time, might the macular ganglion cell layer thin due to the same mechanism?

Why is this important to us:

  • Lower visual field impairment is a strong indicator of posterior parietal brain injury. This is also where the dorsal streams are present. So it is possible that finding a lack of ganglion cells in the upper retina on both eyes will also prove to be a marker for dorsal stream dysfunction, which is very difficult to diagnose.
  • It will not be the case for everyone, but with history taking and developing diagnostic criteria, we are getting a better understanding of how and where some may be affected by CVI.

The CVIs and OVIs may affect each other in ways we are only just finding out about. This is a young subject, and when we learn something new, we have to go back over what we thought we knew, and see where revisions to our knowledge are needed.

Paper: Injuries to the Immature Optic Radiation Show Correlated Thinning of the Macular Ganglion Cell Layer, Finn Lennartsson et al, Frontiers in Neurology, 7 May 2018


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